|
| Status |
Public on Aug 14, 2017 |
| Title |
Pregnant_1st tri_MR_2 |
| Sample type |
RNA |
| |
|
| Source name |
Whole blood
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: Female
pregnancy status: Pregnant - 1st trimester
infection: African ZIKV (MR766)
cell type: CD14+ monocytes
|
| Treatment protocol |
In vitro whole blood infection was performed using different strains of ZIKV. At 40 hpi, peripheral blood mononuclear cells (PBMCs) were isolated using density gradient centrifugation using SepMate and lymphocyte separation medium. Total monocytes were further isolated from PBMCs using Pan Monocyte Isolation Kit.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
10,000 CD14+ sorted monocytes were lysed in RLT buffer at a concentration of 6500 cells/ul.
|
| Label |
N/A
|
| Label protocol |
N/A
|
| |
|
| Hybridization protocol |
N/A
|
| Scan protocol |
Sample Cartridges are placed in the Digital Analyzer for data collection. Color codes on the surface of the cartridge are counted and tabulated for each target molecule. The data produced by the nCounter Digital Analyzer are exported as a Reporter Code Count (RCC) file.
|
| Data processing |
Data collection is performed using epifluorescence microscopy and CCD capture technology on an nCounter instrument to yield hundreds of thousands of target molecule counts. Digital images are processed within the nCounter instrument, and the Reporter Probe counts are tabulated in a comma-separated value (CSV) format or RCC file for downstream data analysis with NanoString’s nSolverTM Analysis Software.
|
| |
|
| Submission date |
Jul 20, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Suan-Sin Foo |
| E-mail(s) |
foos@usc.edu
|
| Organization name |
University of Southern California
|
| Department |
Molecular Microbiology and Immunology
|
| Lab |
Dr. Jae U Jung lab
|
| Street address |
1501 San Pablo St
|
| City |
Los Angeles |
| State/province |
California |
| ZIP/Postal code |
90089 |
| Country |
USA |
| |
|
| Platform ID |
GPL23816 |
| Series (1) |
| GSE101718 |
Immunoprofiling of CD14+ blood monocytes upon Zika virus infection |
|
