|
| Status |
Public on Aug 01, 2018 |
| Title |
Scr-4h |
| Sample type |
RNA |
| |
|
| Source name |
P493-6 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: P493-6
treatment: scramble shRNA, 4h after Tet withdrawl
|
| Treatment protocol |
P493-6 cells were infected with lentiviruses expressing control (scramble) or Dpy30 shRNAs #2 or #5, followed by puromycin (2 μg/ml) selection for 2–3 days starting from 2 days after infection. control and Dpy30 KD P493-6 cells were treated with tetracycline for 3 days (to turn off Myc). Tetracycline was thoroughly washed and cells were incubated in Tet-free medium for 4 hours. RNAs from cells before Tet treatment, after Tet treatment and right before washing Tet away, and 4h after washing Tet away, were subjected to Illumina microarray analyses.
|
| Growth protocol |
P493-6 cells were cultured in RPMI 1640 medium with 10% fetal bovine serum (FBS).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using the RNeasy kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina TotalPrep RNA Amplification kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Data processing |
Only Gene probes with Detection P value below 0.05 in at least one samples were kept for further processing including normalization. Signals were normalized against the median in each sample.
|
| |
|
| Submission date |
Jul 25, 2017 |
| Last update date |
Aug 01, 2018 |
| Contact name |
Hao Jiang |
| E-mail(s) |
haojiang@uab.edu
|
| Organization name |
University of Alabama at Birmingham
|
| Department |
Biochemistry and Molecular Genetics
|
| Street address |
1825 University Blvd
|
| City |
Birmingham |
| State/province |
AL |
| ZIP/Postal code |
35294 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE101852 |
Dpy30 regulates Myc binding to targets and Myc-driven tumorigenesis (Illumina HumanHT-12 V4.0 expression) |
| GSE101854 |
Dpy30 regulates Myc binding to targets and Myc-driven tumorigenesis |
|
