genotype: wild type age: 10-12 weeks Sex: Male diet: Teklad chow tissue: Liver exposure time: 14 days
Treatment protocol
Livers were excised for RNA extraction
Growth protocol
Mice were housed in colony cages in rooms maintained on a 12-h light/12-h dark cycle.
Extracted molecule
total RNA
Extraction protocol
Total RNA was prepared from each liver by using RNA STAT-60 (Tel-Test, Friendswood, TX)
Label
biotin
Label protocol
Equal aliquots of total RNA from each of five mouse livers in each group were pooled (total, 20 μg) and used for biotin labeling as described in the Affymetrix technical bulletin
Hybridization protocol
Hybridization and washing of the Affymetrix GeneChip Murine Genome MU74A, -B, and -C version 2 arrays (Affymetrix, Santa Clara, CA) were carried out as described in: Mahadevappa, M. & Warrington, J. A. (1999) Nat. Biotechnol. 17, 1134–1136. Duplicate hybridizations were performed with each RNA sample.
Scan protocol
Scanning, and analysis of the Affymetrix GeneChip Murine Genome MU74A, -B, and -C version 2 arrays (Affymetrix, Santa Clara, CA) were carried out as described in: Mahadevappa, M. & Warrington, J. A. (1999) Nat. Biotechnol. 17, 1134–1136.
Description
Mice received four i.p. injections of polyinosinic-polycytidylic acid to induce the Cre recombinase and euthanized 14 days after last injection
Data processing
Gene expression results were analyzed by standard methods using Robust Multichip Average (RMA) normalization.
