|
| Status |
Public on Aug 05, 2017 |
| Title |
Liver_Human_Transgenic_SREBP1a_chipB_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Liver, transgenic SREBP-1a mouse
|
| Organism |
Mus musculus |
| Characteristics |
genotype: TgSREBP-1a
age: 12-16 weeks
Sex: Male
diet: high protein/low carbohydrate
tissue: Liver
exposure time: 2 weeks
|
| Treatment protocol |
Livers were excised for RNA extraction
|
| Growth protocol |
Mice were housed in colony cages in rooms maintained on a 12-h light/12-h dark cycle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was prepared from each liver by using RNA STAT-60 (Tel-Test, Friendswood, TX)
|
| Label |
biotin
|
| Label protocol |
Equal aliquots of total RNA from each of five mouse livers in each group were pooled (total, 20 μg) and used for biotin labeling as described in the Affymetrix technical bulletin
|
| |
|
| Hybridization protocol |
Hybridization and washing of the Affymetrix GeneChip Murine Genome MU74A, -B, and -C version 2 arrays (Affymetrix, Santa Clara, CA) were carried out as described in: Mahadevappa, M. & Warrington, J. A. (1999) Nat. Biotechnol. 17, 1134–1136. Duplicate hybridizations were performed with each RNA sample.
|
| Scan protocol |
Scanning, and analysis of the Affymetrix GeneChip Murine Genome MU74A, -B, and -C version 2 arrays (Affymetrix, Santa Clara, CA) were carried out as described in: Mahadevappa, M. & Warrington, J. A. (1999) Nat. Biotechnol. 17, 1134–1136.
|
| Description |
Mice were fed a high protein/low carbohydrate diet for 2 weeks. This diet was used to elicit high-level expression of the nSREBPs
|
| Data processing |
Gene expression results were analyzed by standard methods using Robust Multichip Average (RMA) normalization.
|
| |
|
| Submission date |
Aug 04, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Christopher Corton |
| E-mail(s) |
corton.chris@epa.gov
|
| Organization name |
US-EPA
|
| Lab |
MD B105-01
|
| Street address |
109 TW Alexander Dr.
|
| City |
Research Triangle Park |
| State/province |
NC |
| ZIP/Postal code |
27711 |
| Country |
USA |
| |
|
| Platform ID |
GPL82 |
| Series (2) |
| GSE102257 |
Combined analysis of oligonucleotide microarray data from transgenic and knockout mice identifies direct SREBP target genes [MG_U74Bv2] |
| GSE102259 |
Combined analysis of oligonucleotide microarray data from transgenic and knockout mice identifies direct SREBP target genes |
|
