Data table header descriptions |
ID_REF |
|
CH1_median |
Channel 1 (635 nm, Cy5, Experiment) median pixel intensity |
CH2_median |
Channel 2 (532 nm, Cy3, Reference) median pixel intensity |
CH1_back |
Channel 1 Background |
CH2_back |
Channel 2 Background |
CH1_MEAN |
Channel 1 Mean |
CH2_MEAN |
|
CH1_SD |
Channel 1 Standard Deviation |
CH2_SD |
Channel 2 Standard Deviation |
pixels |
Number of pixels used to determine signal. |
bpixels |
Number of pixels used to determine background. |
x |
X-coordinate of the center of the feature-indicator associated with the feature, where (0,0) is the top left of the image |
y |
Y-coordinate of the center of the feature-indicator associated with the feature, where (0,0) is the top left of the image |
diameter |
diameter in īm of the feature-indicator |
%>CH1_back+1SD |
percentage of feature pixels with intensities more than one standard deviation above the background pixel intensity, for Channel 1 |
%>CH1_back+2SD |
percentage of feature pixels with intensities more than two standard deviations above the background pixel intensity, for Channel 1 |
%>CH2_back+1SD |
percentage of feature pixels with intensities more than one standard deviation above the background pixel intensity, for Channel 2 |
%>CH2_back+2SD |
percentage of feature pixels with intensities more than two standard deviations above the background pixel intensity, for Channel 2 |
CH1_%Sat |
percentage of pixel saturation within feature for Channel 1 |
CH2_%Sat |
percentage of pixel saturation within feature for Channel 2 |
QUALITY |
|
flag |
An extension of GenePix 3.0 flags described at http://twiki.med.yale.edu/twiki/bin/view/YMD/YMDFlagFields |
ratio_calc |
(CH1_median-CH1_back)/(CH2_median-CH2_back) |
VALUE |
log ratio (log2 of PRE_VALUE) |
norm_factor |
Normalization factor calculated as described at http://twiki.med.yale.edu/twiki/bin/view/YMD/NormalizationAndFilterMethods |
PRE_VALUE |
[(Experiment channel median intensity - background)/(Reference channel median intensity - background)]*(norm_factor) |