|
| Status |
Public on Jul 19, 2018 |
| Title |
Middle cerebral artery occlusion (MCAO)_24h_repicate_4 |
| Sample type |
RNA |
| |
|
| Source name |
Middle cerebral artery occlusion (MCAO) 24h
|
| Organism |
Mus musculus |
| Characteristics |
treatment: MCAO
time: 1
|
| Treatment protocol |
For the microarray study hearts were isolated from four study groups and their respective controls: 1) hearts from C57BL/6J animals 24h after occlusion of the middle cerebral artery, 2) hearts from C57BL/6J animals 72h after occlusion of the middle cerebral artery, 3) hearts from C57BL/6J animals, 24h after sham operation and 4) hearts from C57BL/6J 72h after sham operation.
|
| Growth protocol |
The study was performed with 8 weeks old C57BL/6J animals, exposed to either sham operation or occlusion of the medial cerebral artery. All mice had free access to food and water. Hearts were isolated 24h and 72h after occlusion of the medial cerebral artery. As control, age matched sham operated C57BL/6J mice were used.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000
|
| Description |
Heart tissue from C57BL/6J animals, 24h after ischemic stroke of the brain
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 7, from SAS. Gene expression profiling was performed using arrays of mouse Mogene-2_0-type from Affymetrix. Additional analysis was performed with R and HOMER (transcription factor binding motif finder)
|
| |
|
| Submission date |
Aug 11, 2017 |
| Last update date |
Jul 19, 2018 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL20710 |
| Series (1) |
| GSE102558 |
Expression data from heart tissue after induction of ischemic stroke (occlusion of the middle cerebral artery) |
|
