|
| Status |
Public on Jul 19, 2018 |
| Title |
TFK-1 with miR-145, replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
miR-145 mimic-transfected bile duct carcinoma cells TFK-1
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: TFK-1
cell type: bile duct carcinoma cell line
treatment: miR-145-5p mimic
|
| Treatment protocol |
TFK-1 cells were transfected using RNAiMAX for miRNA mimics or Lipofectamine 2000 transfection reagent for plasmid DNA (Life Technologies) according to the manufacturer's instructions.
|
| Growth protocol |
TFK-1 were maintained in RPMI-1640 medium. Media was supplemented with 10% fetal calf serum and 1% Penicillin-streptomycin (100 IU/ml and 100 g/ml, respectively).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA of cell lines was isolated using TRIzol Reagent (Invitrogen, Life Technologies, Carlsbad, CA) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Hybridization (16h x 45°C) was processed according to the standard Affymetrix protocol.
|
| Scan protocol |
Affymetrix GeneArray Scanner3000.
|
| Description |
TFK_1_145_2
TFK-1 cells were transfected using RNAiMAX for miR-145 mimics.
|
| Data processing |
The data were analyzed with a commercial software called JMP Genomics, version 7, from SAS. Gene expression profiling was performed using arrays of human HTA-2_0-type from Affymetrix. A Custom CDF Version 20 with Entrez based gene definitions was used to annotate the arrays. The Raw fluorescence intensity values were normalized applying quantile normalization, RMA background correction and Medianpolish Probeset Summary.
|
| |
|
| Submission date |
Aug 18, 2017 |
| Last update date |
Jul 19, 2018 |
| Contact name |
Carsten Sticht |
| Organization name |
University Heidelberg
|
| Department |
ZMF
|
| Street address |
Theodor-Kutzer-Ufer
|
| City |
Mannheim |
| ZIP/Postal code |
68169 |
| Country |
Germany |
| |
|
| Platform ID |
GPL22936 |
| Series (1) |
| GSE102815 |
miRNA-145 signalling in TFK-1 cells |
|
