|
| Status |
Public on Feb 13, 2018 |
| Title |
HUVECs non-infected-24h-3 |
| Sample type |
RNA |
| |
|
| Source name |
HUVECs non-infected with influenza A virus
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: human umbilical vein endothelial cells (HUVECs)
infected with: none (uninfected)
time point: 24h
|
| Treatment protocol |
When grown to 90% confluency, HUVECs were removed from the culture medium and washed twice with PBS. Cells were infected with influenza A virus in serum-free media at a multiplicity of infection (MOI) of 20~40 and harvested at the indicated time points. The non-infected cells were used as normal control.Total RNA was extracted from uninfected and infected HUVECs using Trizol reagent (Invitrogen) following the manufacturer’s protocol. RNA pellets were resuspended in RNase-free water. RNA quantification was performed according to the Affymetrix recommended protocols.
|
| Growth protocol |
Type Culture Collection (ATCC) and maintained in Endothelial Cell Medium (ECM, Sciencecell) supplemented with 1% Endothelial Cell Growth supplements (ECGS), 5% fetal calf serum serum (FCS, Sigma), 1% penicillin and 1% streptomycin. Madin Darby canine kidney (MDCK) cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, GIBCO) supplemented with 10% fetal bovine serum (FBS, Sigma), 1% penicillin and 1% streptomycin. The cells were cultured in a humidified atmosphere of 5% CO2 at 37℃.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
RNA quantification was performed according to the Affymetrix recommended protocols. Total RNA was labeled using the FlashTag™ Biotin RNA Labeling Kit
|
| |
|
| Hybridization protocol |
Briefly, the process begins with a brief poly(A) tailing reaction followed by ligation of the biotinylated signal molecule to the target RNA sample. The labeled miRNA was hybridized to Affymetrix® GeneChip® miRNA (Affymetrix miRNA 4.0) according to the manufacturer’s protocols.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner (GCS) 3000 7G.
|
| Description |
HUVECs non-infected-24h-3
|
| Data processing |
The data were analyzed with Expression Console version 1.4(RMA + DABG analysis) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Aug 21, 2017 |
| Last update date |
Feb 13, 2018 |
| Contact name |
Wu Ying |
| E-mail(s) |
aqiwuying@hotmail.com
|
| Organization name |
Beijing University of Chinese Medicine
|
| Department |
Department of Microbiology and Immunology
|
| Lab |
Department of Microbiology and Immunology
|
| Street address |
No. 11, Bei San Huan Dong Lu, Chaoyang District
|
| City |
Beijing |
| ZIP/Postal code |
100078 |
| Country |
China |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE102866 |
Expression data from HUVECs infected with influenza A virus PR8 and CA07 |
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