|
| Status |
Public on Oct 30, 2017 |
| Title |
pDAB447-030 T3 (pool) |
| Sample type |
SRA |
| |
|
| Source name |
Maize leaves, V5/V6
|
| Organism |
Zea mays |
| Characteristics |
tissue: leaf
transgene construct: pDAB
copy number of transgene: single copy
multigene construct design: cry1Ab, ZmHiGC
|
| Growth protocol |
Greenhouse grown plants
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction
small RNA library preparation
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
sRNA
Pooled tissue sample:111447[1]-030.AJ001.Sx004.Sx018.156+111447[1]-030.AJ001.Sx004.Sx018.169+111447[1]-030.AJ001.Sx004.Sx018.170+111447[1]-030.AJ001.Sx004.Sx018.171+111447[1]-030.AJ001.Sx004.Sx018.173111447[1]-030.AJ001.Sx004.Sx018.174
|
| Data processing |
For data processing, the adapter sequences were trimmed from raw sequence reads. The small RNA sequences were further trimmed to the lengths between 14 nt and 35 nt nucleotides (for HiSeq 2000) and 14 nt and 75 nt (for Nextseq 500).
Sequence data processing was conducted using in-house Perl scriptsand Unix shell scripts developed by Genomics group at DOW AgroSciences.
Genome_build: Maize B73 AGPv2_5b
Supplementary_files_format_and_content: tag count file
|
| |
|
| Submission date |
Aug 24, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Lyudmila V Sidorenko |
| Organization name |
DOW AgroSciences
|
| Street address |
9330 Zionsville Rd
|
| City |
Indianapolis |
| State/province |
Indiana |
| ZIP/Postal code |
46268 |
| Country |
USA |
| |
|
| Platform ID |
GPL20156 |
| Series (2) |
| GSE103037 |
GC-rich coding sequences reduce transposon-like sRNA-mediated transgene silencing in Arabidopsis and maize |
| GSE103112 |
GC-rich coding sequences reduce transposon-like sRNA-mediated transgene silencing in Arabidopsis and maize |
|
| Relations |
| BioSample |
SAMN07551981 |
| SRA |
SRX3124539 |
