|
| Status |
Public on Jun 26, 2008 |
| Title |
Thymic lymphoma NRH5-4 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Total cDNA from wild-type thymus
|
| Organism |
Mus musculus |
| Characteristics |
Wild-type
|
| Treatment protocol |
4-week-old mice of both sexes were exposed to four weekly doses of 1.75 Gy/dose of ionizing gamma
|
| Growth protocol |
Tumors develop in vivo after irradiation of animals
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total DNA was extracted by routine phenol extraction. The quality of the DNA was evaluated by gel electrophoresis.
|
| Label |
Cy3
|
| Label protocol |
One microgram of test and reference DNA were labeled with fluorescent Cy5 and Cy3 (Amhersam, Sunnyvale), respectively.
|
| |
|
| Channel 2 |
| Source name |
Thymic lymphoma NRH5-4
|
| Organism |
Mus musculus |
| Characteristics |
Wild-type
|
| Treatment protocol |
4-week-old mice of both sexes were exposed to four weekly doses of 1.75 Gy/dose of ionizing gamma
|
| Growth protocol |
Tumors develop in vivo after irradiation of animals
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Total DNA was extracted by routine phenol extraction. The quality of the DNA was evaluated by gel electrophoresis.
|
| Label |
Cy5
|
| Label protocol |
One microgram of test and reference DNA were labeled with fluorescent Cy5 and Cy3 (Amhersam, Sunnyvale), respectively.
|
| |
|
| |
| Hybridization protocol |
Hybridizations were performed at 55°C for 17 h using the SlideHyb Microarray Hybridization buffer (Ambion).
|
| Scan protocol |
the slides were scanned in an Agilent G2565BA scanner.
|
| Description |
Tumor DNA versus wild-type DNA
|
| Data processing |
Images were then analyzed with GenePix 5.1 (Axon Instruments, Inc., Union City, CA) and the TM4 software
|
| |
|
| Submission date |
Mar 17, 2008 |
| Last update date |
Jun 26, 2008 |
| Contact name |
Marcos Malumbres |
| E-mail(s) |
mmm@cnio.es
|
| Organization name |
Centro Nacional de Investigaciones Oncológicas (CNIO)
|
| Department |
Molecular Oncology
|
| Lab |
Cell Division and Cancer
|
| Street address |
Melchor Fernandez Almagro 3
|
| City |
Madrid |
| ZIP/Postal code |
E-28029 |
| Country |
Spain |
| |
|
| Platform ID |
GPL6592 |
| Series (2) |
| GSE10858 |
Comparative Genomic hybridization of gamma-irradiated T-cell lymphomas |
| GSE10861 |
T-cell lymphoblastic lymphomas induced by gamma-irradiation |
|
