|
| Status |
Public on Mar 18, 2008 |
| Title |
flu_60min |
| Sample type |
RNA |
| |
|
| Source name |
Rosette leaves of the flu mutant (Ler) grown under continuous light, transferrd to the dark for 8h and shifted to light for 60 min.
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Landsberg erecta (Ler)
Genotype: flu1-1
Plant material : rosette leaves of 3 week-old plants grown under continuous light 90 mmol. m-2 . s-1, before bolting
|
| Treatment protocol |
Plants were transferred to the dark for 8 h and rosette leaves were harvested at 30 min, 60 min, and 2 h after reillumination.
|
| Growth protocol |
Plants were grown on soil for 3 weeks under continuous light at 90 mmol. m-2 . s-1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA extraction was performed according to the guanidine-hydrochloride method as described in Melzer, S., Majewski, D. M. & Apel, K. (1990) Plant Cell 2, 953-961.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 20 ug total RNA (Eukaryotic Target Preparation, 2002, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Arabidopsis ATH1 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400, protocol EukGE-WS2v4.
|
| Scan protocol |
GeneChips were scanned using the Agilent GeneArray Scanner
|
| Description |
Gene expression data from rosette leaves of 3-week-old flu mutant plants (Ler) grown under continuous light, 60min after a 8hdark/light shift
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
|
| |
|
| Submission date |
Mar 18, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Christophe Laloi |
| E-mail(s) |
claloi@ethz.ch
|
| Phone |
+41 44 632 63 58
|
| Fax |
+41 44 632 10 81
|
| URL |
http://www.pg.ethz.ch/people/laloic
|
| Organization name |
ETH Zurich
|
| Department |
Institute of Plant Science
|
| Lab |
LFW D17.2
|
| Street address |
Universitaetstrasse 2
|
| City |
Zurich |
| ZIP/Postal code |
8092 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE10876 |
Expression data from Arabidopsis thaliana (Ler) rosette leaves after the release of singlet oxygen inside plastids |
|
| Relations |
| Reanalyzed by |
GSE119083 |
