|
| Status |
Public on Feb 17, 2019 |
| Title |
Nasal swab specimen from child with Acute asthma/wheeze [MAV030] |
| Sample type |
RNA |
| |
|
| Source name |
Nasal swab specimen
|
| Organism |
Homo sapiens |
| Characteristics |
phenotype: Acute asthma/wheeze
rv status: Rhinovirus negative
|
| Treatment protocol |
Nasal swab specimens were collected using flocked swabs.
|
| Growth protocol |
Samples were collected from children during a naturally occuring asthma or wheezing exacerbation.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with TRIzol followed by RNeasy.
|
| Label |
Biotin
|
| Label protocol |
Standardized kits from Affymetrix were used to label the samples at the Ramaciotti Centre for Genomics, The University of New South Wales.
|
| |
|
| Hybridization protocol |
The samples were hybridized to Human Gene 2.1 ST microarrays according to standard Affymetrix protocols. This was performed by at the Ramaciotti Centre for Genomics, The University of New South Wales.
|
| Scan protocol |
The samples were scanned on an Affymetrix scanner at the Ramaciotti Centre for Genomics, The University of New South Wales.
|
| Data processing |
The raw microarray data was processed in R employing the RMA algorithm. A custom chip description file was used (hugene21sthsentrezg, version 20).
|
| |
|
| Submission date |
Aug 28, 2017 |
| Last update date |
Feb 17, 2019 |
| Contact name |
Anthony Bosco |
| E-mail(s) |
Anthony.Bosco@telethonkids.org.au
|
| Organization name |
Telethon Kids Institute
|
| Street address |
100 Roberts Road
|
| City |
Subiaco |
| State/province |
WA |
| ZIP/Postal code |
6008 |
| Country |
Australia |
| |
|
| Platform ID |
GPL23961 |
| Series (1) |
| GSE103166 |
Gene expression profiling of nasal swabs samples from children with asthma or wheezing exacerbations. |
|
