|
| Status |
Public on Sep 27, 2017 |
| Title |
D4 |
| Sample type |
RNA |
| |
|
| Source name |
Human_Ad5_24h_donor 1
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: monocyte-derived dendritic cells
source: Human PBMCs
|
| Treatment protocol |
Monocyte-derived dendritic cells were infected with ALVAC or Ad5 empty viral vectors (MOI=3) for 24 hours.
|
| Growth protocol |
Monocytes were isolated from health PBMCs with EasySep™ Human CD14 Positive Selection Kit II according to the manufacture's protocol. Then isolated monocytes were induced to differentiate to dendritic cells under the treatment of human recombinant IL-4 and GM-CSF for 5 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Quick-RNA™ MicroPrep Kit following the manufacture's protocol. Quality control was performed with Agilent Bioanalyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 1
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Aug 28, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Fengliang Liu |
| E-mail(s) |
fenliu@utmb.edu
|
| Phone |
409-772-0926
|
| Organization name |
The University of Texas Medical Branch
|
| Department |
Microbiology and Immunlogy
|
| Street address |
301 University Blvd
|
| City |
Galveston |
| State/province |
Texas |
| ZIP/Postal code |
77555 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE103204 |
The comparsion of gene expression in ALVAC- and Ad5-infected human monocyte-derived dendritic cells |
|
