|
| Status |
Public on Nov 06, 2017 |
| Title |
231_1a3_nt_rep1 [methylation] |
| Sample type |
genomic |
| |
|
| Source name |
MDA-MB-231 1a3 NT
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB-231
vector: MSCV ALDH1A3 overexpression
atra: 0 nM
decitabine: 0 uM
replicate: 1
|
| Treatment protocol |
atRA and decitabine as inidcated.
|
| Growth protocol |
MDA-MB-231 and MDA-MB-468 cells grown in DMEM with 10% FBS, 1x antibiotic-antimycotic, 0.25 ug/mL puromycin.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
DNA extracted with the PureLink DNA kit (Invitrogen)
|
| Label |
cy3, cy5
|
| Label protocol |
as described in Illumina protocol
|
| |
|
| Hybridization protocol |
as described in Illumina protocol
|
| Scan protocol |
as described in Illumina protocol
|
| Description |
1A3 NT 1
|
| Data processing |
as described in Illumina protocol
|
| |
|
| Submission date |
Sep 04, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Paola Marcato |
| Organization name |
Dalhousie University
|
| Department |
Pathology
|
| Street address |
PO Box 15000
|
| City |
Halifax |
| State/province |
Nova Scotia |
| ZIP/Postal code |
B3H 4R2 |
| Country |
Canada |
| |
|
| Platform ID |
GPL13534 |
| Series (2) |
| GSE103425 |
DNA methylation profiling of MDA-MB-231 and MDA-MB-468 after ALDH1A3 manipulation, all-trans retinoic acid treatment, decitabine treatment |
| GSE103427 |
Profiling of the transcriptional response to all-trans retinoic acid in breast cancer cells reveals RARE-independent mechanisms of gene expression |
|
