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| Status |
Public on Apr 29, 2019 |
| Title |
FB23-2-rep2 |
| Sample type |
SRA |
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| Source name |
NB4
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| Organism |
Homo sapiens |
| Characteristics |
cell line: NB4
cell type: acute monocytic leukemia
treatment: FTO inhibition
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| Extracted molecule |
polyA RNA |
| Extraction protocol |
RNA was extracted by using TRIzol reagent (Invitrogen, Carlsbad, CA). NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs, Ipswich, MA) was used for polyA RNA purification.
Library was prepared by or NEBNext Ultra Directional RNA Library Prep Kit (New England BioLabs, Ipswich, MA).
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 1000 |
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| Description |
rep2
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| Data processing |
sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence
the RPKM were calculated using RSEM 1.2.31 with default parameters
Genome_build: GRCh38
Supplementary_files_format_and_content: RSEM output file containing the expression of genes
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| Submission date |
Sep 05, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Jianjun Chen |
| E-mail(s) |
jianchen@coh.org
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| Phone |
6262185591
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| Organization name |
Beckman Research Institute of City of Hope
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| Department |
Systems Biology
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| Lab |
Chen Lab
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| Street address |
1218 S. 5th Ave., Room 2062
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| City |
Monrovia |
| State/province |
CA |
| ZIP/Postal code |
91016 |
| Country |
USA |
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| Platform ID |
GPL15433 |
| Series (2) |
| GSE103495 |
RNA seq with AML (NB4) cells upon FTO inhibition |
| GSE103497 |
RNA-seq and m6A-seq of AML cells with FTO knockdown or inhibition |
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| Relations |
| BioSample |
SAMN07605624 |
| SRA |
SRX3161632 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM2772469_232.2.genes.results.txt.gz |
443.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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