|
| Status |
Public on Sep 08, 2017 |
| Title |
Human ES cell line-KhES-1-E8 feeder-free |
| Sample type |
genomic |
| |
|
| Source name |
Human ES cell line KhES-1
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: embryonic stem cell
cell line: KhES-1
culture medium: E8
culture condition: feeder-free
|
| Growth protocol |
AKIT or Essential 8 medium on 0.5µg/cm2 Laminin-E8 fragment-coated dishes, or standard KSR/bFGF medium on MEF feeder layer
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Proteinase K and Rnase I treatment followed by phenol-chloroform extraction and isopropanol precipitation
|
| Label |
biotin
|
| Label protocol |
As per manufacturer (Affymetrix)
|
| |
|
| Hybridization protocol |
GeneChip hybridization oven 645 as per manufacturer (Affymetrix)
|
| Scan protocol |
GeneChip Scanner 3000 7G System
|
| Data processing |
CNV, LOH and Loss or Gain Mosaic was anlyzed by Single Sample Anlysis NA33 in Affimetrix Chromosome Analysis Suite 3.1 with smoothing window of 0 Mb
CNV, LOH and Mosaic segements detected with filter of 0 marker count and 0kb size
|
| |
|
| Submission date |
Sep 08, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Kouichi Hasegawa |
| E-mail(s) |
hasegawa-k@sahs.med.osaka-u.ac.jp
|
| Organization name |
Osaka University
|
| Department |
StemRIM Institute of Regeneration-Inducing Medicine
|
| Lab |
Drug Efficacy Evaluation & Pharmacology Dept. StemRIM
|
| Street address |
6F Techno Alliance Bldg. 2-8 Yamadaoka
|
| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
| |
|
| Platform ID |
GPL18637 |
| Series (2) |
| GSE103636 |
Human embryonic stem cells in AKIT, E8 and KSR/bFGF culture medium |
| GSE103669 |
Growth factor-free, chemically-defined culture system for expansion and derivation of human pluripotent stem cells |
|
