|
| Status |
Public on Sep 11, 2020 |
| Title |
CtrlO_2 |
| Sample type |
SRA |
| |
|
| Source name |
Embryo
|
| Organism |
Drosophila melanogaster |
| Characteristics |
developmental stage: 20-23 hours after egg laying
genotype: Tdc2-Gal4;UAS-mCherry
tissue: Embryo
|
| Growth protocol |
Flies were maintained on apple juice plates supplemented with fresh yeast. Embryos were collected for three hours at 25°C and subsequently developed for 13 hours for the neuronal control and 20 hours for the octopaminergic control. Cells were isolated, FACS sorted and collected in lysis buffer.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Embryonic cells were isolated as described before (Salmand & Perrin, 2011), FACS sorted and 10 cells per replicate were collected in lysis buffer and snap-frozen at -80°C.
Libary preparation was done using the Smart-Seq 2 Kit for Illumina
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
Octopaminergic mCherry expression
|
| Data processing |
The libraries were sequenced on NextSeq500 with 76 bp single read
After sequencing reads were mapped using STAR (v. 2.5.2b) against an rRNA refence and unmapped reads were mapped against ensembl release 87 (BDGP6).
normalized bigwig tracks were created using samtools (v.1.3.1), bedtools (2.25.0) and kentUtils (v302).
Genome_build: BDGP6
Supplementary_files_format_and_content: read depth normalized bigwig tracks (bw)
|
| |
|
| Submission date |
Sep 11, 2017 |
| Last update date |
Sep 11, 2020 |
| Contact name |
Jean-Yves Roignant |
| Organization name |
Institute of molecular Biology
|
| Lab |
Roignant
|
| Street address |
Ackermannweg 4
|
| City |
Mainz |
| ZIP/Postal code |
55128 |
| Country |
Germany |
| |
|
| Platform ID |
GPL19132 |
| Series (2) |
| GSE103701 |
RNA-Sequencing of FACS sorted neurons and octopaminergic cells |
| GSE103710 |
Roles of Lola-O |
|
| Relations |
| BioSample |
SAMN07629607 |
| SRA |
SRX3176366 |
