|
| Status |
Public on Sep 13, 2017 |
| Title |
Unstimulated Neutrophils donor 4 |
| Sample type |
RNA |
| |
|
| Source name |
Human primary Neutrophils donor 4
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: blood
cell type: human primary neutrophils
donor: 1
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy3
|
| Label protocol |
RNA labeling was done with the one-color Low Input Quick Amp Labeling Kit (Agilent Technologies).
|
| |
|
| Hybridization protocol |
mRNA was reverse transcribed and amplified using an oligo-dT-T7 promoter primer, T7 RNA Polymerase and Cyanine 3-CTP. After precipitation, purification, and quantification, 0.75 μg labeled cRNA was fragmented and hybridized to custom-commercial in situ oligonucleotide whole genome human 8 × 60k multipack microarrays (Agilent-048908) according to the supplier’s protocol (Agilent Technologies).
|
| Scan protocol |
Scanning of microarrays was carried out with 3 μm resolution using a G2565CA high-resolution laser microarray scanner (Agilent Technologies
|
| Data processing |
Microarray image data were analyzed with the Image Analysis/Feature Extraction software G2567AA v. A.11.5.1.1 (Agilent Technologies) using default settings and the GE1_1105_Oct12 extraction protocol.
|
| |
|
| Submission date |
Sep 12, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Brian Caffrey |
| E-mail(s) |
caffrey@molgen.mpg.de
|
| Organization name |
Max Planck Institute for molecular Genetics
|
| Street address |
Ihnestrasse 63-73
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
| |
|
| Platform ID |
GPL21272 |
| Series (1) |
| GSE103755 |
Cell Cycle Proteins Control Production of Neutrophil Extracellular Traps (NETs) |
|
