|
| Status |
Public on Sep 14, 2017 |
| Title |
Thymus sample 11, Grade 0 |
| Sample type |
RNA |
| |
|
| Source name |
myasthenia gravis thymus
|
| Organism |
Homo sapiens |
| Characteristics |
disease state: myasthenia gravis
tissue: Thymus
grade: Grade 0
morphology: No Germinal Center
|
| Treatment protocol |
N/A
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from thymus blocks using mirVana™ miRNA isolation kit (Ambion) according to the manufacturer’s protocol. The RNA samples were treated with RNase Free DNase (QIAGEN, Valencia, CA) and were purified using RNeasy mini kit (QIAGEN) according to the manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
250ng of total RNA was labeled by FlashTag™ Biotin HSR RNA Labeling Kit (Affymetrix, Inc., Santa Clara, CA).
|
| |
|
| Hybridization protocol |
Labeled samples with eukaryotic hybridization control were hybridized to GeneChip® miRNA 4.0 Array (Affymetrix) chips in a Gene Chip hybridization Oven 640 (Affymetrix) for 18hrs.
|
| Scan protocol |
These were then scanned by an Affymetrix GeneChip Scanner (GC30007G).
|
| Description |
miRNA expression data
|
| Data processing |
CHP file was generated by Expression Console (Affymetrix). Array data was normalized, batch corrected and analyzed (1-way ANOVA) using Partek Genomics Suite program.
|
| |
|
| Submission date |
Sep 13, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Manjistha Sengupta |
| Organization name |
George Washington University
|
| Department |
Neurology
|
| Street address |
2300 Eye Street NW, Ross Hall, Room #723
|
| City |
Washington DC |
| State/province |
District of Columbia |
| ZIP/Postal code |
20037 |
| Country |
USA |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE103812 |
miRNA expression profiling of thymus samples from myasthenia gravis patients |
|
