|
Status |
Public on Apr 12, 2018 |
Title |
NHP_8_DPI_6 |
Sample type |
SRA |
|
|
Source name |
Whole Blood
|
Organism |
Macaca fascicularis |
Characteristics |
tissue: Whole Blood agent: Ebola virus Makona route: IN MAD dpi: 6
|
Treatment protocol |
12 Cynomolgus Macaques infected with EBOV-Makona through intranasal routes
|
Extracted molecule |
total RNA |
Extraction protocol |
500ul whole blood mixed with 500 ul of TRIzol LS and purified using the PureLink RNA MiniKit Constructed on a Sciclone G3 Liquid Handling Robot with TruSeq Stranded Total RNA Library Prep Kit
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Data processing |
Sequenced on a HiSeq 2500 with paired-end 100bp reads in dual-index format Reads were filtered with FASTX Toolkit v0.0.14 Aligned to Genome using Bowtie 2 v2.2.6 and Tophat v2.1.0 Count Tables Generated using HTSeq v0.5.4 and Python v.2.7.3 Genome_build: Cynomolgus Macques Genome v5.0 Supplementary_files_format_and_content: Text files with raw count values from HTSeq
|
|
|
Submission date |
Sep 13, 2017 |
Last update date |
May 15, 2019 |
Contact name |
Emily Speranza |
E-mail(s) |
sperane@ccf.org
|
Organization name |
Cleveland Clinic Lerner Research Institute
|
Department |
Florida Research and Innovation Center
|
Street address |
9801 SW Discovery Wy
|
City |
Port Saint Lucie |
State/province |
FL |
ZIP/Postal code |
34987 |
Country |
USA |
|
|
Platform ID |
GPL20302 |
Series (1) |
GSE103825 |
A conserved transcriptional response to intranasal Ebola virus exposure in nonhuman primates before onset of fever |
|
Relations |
BioSample |
SAMN07638653 |
SRA |
SRX3182634 |