Col-0 wild-type Arabidopsis thaliana grown at 22C, 16hr/8hr light/dark for ~60 days. Inflorescence tissue was harvested and immediately frozen.
Extracted molecule
polyA RNA
Extraction protocol
Total RNA isolated using Trizol per manufacturer's instructions. Subsequent polyA purification was performed using oligo dT agarose beads (Qiagen) per manufacturer's protocol.
Label
not applicable
Label protocol
not applicable
Hybridization protocol
not applicable
Scan protocol
not applicable
Description
Sequences are 20 or 21 nt tags representing the 5' ends of uncapped, polyA+ RNAs. Library #1 derived from 454 pyrosequencing. Details described in Addo-Quaye et al. (2008)
Data processing
Adapter sequences were computationally removed, and unique tags of 20 or 21nt length were counted. Counts are the raw number of times each sequence was isolated. For simplicity, tags are given in DNA form (i.e. T instead of U).
Raw fasta file prior to signature extraction from adapter regions, linked below as supplementary file.
