|
| Status |
Public on Feb 01, 2018 |
| Title |
Het-mStromal-SVF-FSK |
| Sample type |
SRA |
| |
|
| Source name |
Primary SVF cells from bone stromal cells
|
| Organism |
Mus musculus |
| Characteristics |
genotype: CRTC2-/- CRTC3-/+ mice
tissue: Primary bone marrow stromal SVF cells
treatment: 2 hours FSK treatment
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
total RNA extracted with Qiagen Rneasy kit
polyA RNA isolated and library prepared with NEBnext Ultra kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina MiSeq |
| |
|
| Description |
q4
|
| Data processing |
MiSeq Generation of fastq files
tophat2 alignment
cuffdiff alignment
Genome_build: mm10
Supplementary_files_format_and_content: .diff file from cuffdiff comparing all four samples
|
| |
|
| Submission date |
Sep 19, 2017 |
| Last update date |
May 15, 2019 |
| Contact name |
Ezra Wiater |
| E-mail(s) |
wiater@salk.edu
|
| Phone |
(858)453-4100
|
| Organization name |
Salk Institute For Biological Studies
|
| Department |
PBL
|
| Lab |
Montminy
|
| Street address |
10010 N Torrey Pines Rd
|
| City |
San Diego |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL16417 |
| Series (1) |
| GSE103999 |
CREB Coactivators CRTC2 and CRTC3 Modulate Bone Marrow Hematopoiesis |
|
| Relations |
| BioSample |
SAMN07666117 |
| SRA |
SRX3197471 |
