|
| Status |
Public on Dec 01, 2017 |
| Title |
Day 2 CD4 Tmem cells_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Resident memory CD4 T cells
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6 Foxp3-GFP
age: 6-8 weeks
cell type: Resident memory CD4 T cells
|
| Treatment protocol |
CD4 T cells from peripheral and mesenteric lymph nodes of C57BL/6 Foxp3-GFP mice were purified by using Dynabeads Untouched Mouse CD4 Cells Kit (Invitrogen). Then, CD4 Treg and Tmem cells were flow-cytometry sorted as CD4+ CD8α- TCRβ+ GFP+ for Treg and CD4+ CD8α- TCRβ+ GFP- CD44hi for Tmem using a FACS-ARIA3 flow cytometer.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini kit (QIAGEN) according to manufacturer's manual.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Nugen Ovation PicoV2 protocol from 900 pg total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 3.6 ug of cDNA were hybridized for 17 hr at 45C on GeneChip Mouse Gene 2.0 ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GC3000 Scanner.
|
| Data processing |
Data were normalized using RMA and statistical analysis was realized on Partek Genomics Suite. CDF file ENTREZG V20 was used for annotation.
|
| |
|
| Submission date |
Sep 19, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Franck Letourneur |
| E-mail(s) |
franck.letourneur@inserm.fr
|
| Organization name |
INSERM
|
| Department |
U1016
|
| Lab |
genomic
|
| Street address |
22 rue mechain
|
| City |
Paris |
| ZIP/Postal code |
75014 |
| Country |
France |
| |
|
| Platform ID |
GPL22598 |
| Series (1) |
| GSE104011 |
Characterization with age of resident T cells within secondary lymphoid organs in the steady state |
|
