|
| Status |
Public on Jul 07, 2009 |
| Title |
Central memory, donor 12405 |
| Sample type |
RNA |
| |
|
| Source name |
Central memory, donor 12405
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Central memory
sample type: purified cells
fractional abundance: 0.1193
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNeasy Mini Kit (Qiagen)
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
cRNA was hybridized on GeneChip HGU133 plus v2.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data on Central memory cells from donor 12405
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Apr 04, 2008 |
| Last update date |
Nov 04, 2016 |
| Contact name |
Alex Abbas |
| E-mail(s) |
abbas@gene.com
|
| Organization name |
Genentech, Inc.
|
| Department |
Bioinformatics
|
| Street address |
1 DNA Way
|
| City |
South San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94080 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE11057 |
Memory T Cell Subsets |
| GSE11103 |
Study of human immune and memory T cells using microarray |
|
| Relations |
| Reanalyzed by |
GSE49910 |
| Reanalyzed by |
GSE86362 |
