|
| Status |
Public on Sep 28, 2017 |
| Title |
Tr.M354_1 |
| Sample type |
RNA |
| |
|
| Source name |
Spleen
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Spleen
age: 6 Weeks Old
Sex: Male
strain: C57Bl/6
genotype: Foxp3(M354/M354)
phenotype markers: CD4+TCRb+CD25+
amplification technique: Ambion WT Expression Kit
separation method: Dissection / Trimming
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TriZol
|
| Label |
Biotin
|
| Label protocol |
Affymetrix GeneChip WT Terminal Labeling and Controls Kit
|
| |
|
| Hybridization protocol |
Ambion WT Expression Kit
|
| Scan protocol |
Affymetrix GeneChip Scanner
|
| Description |
Foxp3(M354/M354) Tregs
|
| Data processing |
The datasets was pre-filtered to keep only those probesets for which a gene symbol could be found in the Affymetrix annotation. CEL files were normalized using Affymetrix Power Tools on the predefined probeset ID list mentioned above, and using the standard RMA workflow (background adjustment, quantile normalization, median polish probeset summarization). Output on 2^ scale.
|
| |
|
| Submission date |
Sep 27, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
CBDM Lab |
| E-mail(s) |
cbdm@hms.harvard.edu
|
| Phone |
617-432-7747
|
| Organization name |
Harvard Medical School
|
| Department |
Microbiology and Immunobiology
|
| Lab |
CBDM
|
| Street address |
77 Avenue Louis Pasteur
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE104345 |
FoxP3 scanning mutagenesis reveals functional variegation and mild mutations with atypical autoimmune phenotypes [microarray] |
| GSE104346 |
FoxP3 scanning mutagenesis reveals functional variegation and mild mutations with atypical autoimmune phenotypes |
|
