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Status
Public on Jan 01, 2005
Title
GAP_pH_2A
Sample type
RNA
Channel 1 Source name
Aspergillus parasiticus (pH4.5 growth)
Organisms
Aspergillus flavus ; Aspergillus parasiticus Extracted molecule
total RNA
Channel 2 Source name
Aspergillus parasiticus (pH8.0 growth)
Organisms
Aspergillus flavus ; Aspergillus parasiticus Extracted molecule
total RNA
Description
RNA was isolated from lyophilized cultures of Aspergillus parasiticus (SUI) using Trizol (Life Technologies, Rockville, MD) according to the manufacturers instructions. cDNA synthesis, labeling, and hybridization were performed using a protocol modified from Chhabra, et al., 2003. J Biol Chem 278:7540. pH4.5 growth supports aflatoxin (AF) production, whereas pH8.0 growth supresses AF production. If Value=undefined then Value was set to 0 and FLAG was set to 1.
Submission date
Aug 05, 2004
Last update date
Jun 16, 2005
Contact name
Michael S Price
E-mail(s)
msprice2@liberty.edu Phone
434-592-7277
Organization name
Liberty University
Department
Biology & Chemistry
Street address
1971 University Blvd.
City
Lynchburg
State/province
VA
ZIP/Postal code
24515
Country
USA
Platform ID
GPL1393 Series (1)
Data table header descriptions ID_REF
CH1I Raw signal intensity for channel 1
CH1B Median background intensity for channel 1
CH1AB Mean background intensity for channel 1
CH2I Raw signal intensity for channel 2
CH2B Median background intensity for channel 2
CH2AB Mean background intensity for channel 2
CH2/CH1 Ratio of (CH2I-CH2B)/(CH1I-CH1B)
VALUE log2 transformation of CH2/CH1
FLAG Questionable data points, FLAG=1
Data table ID_REF CH1I CH1B CH1AB CH2I CH2B CH2AB CH2/CH1 VALUE FLAG 1
683
106
161
1184
57
109
1.953
0.965691949
0
2
2304
109
169
2464
61
122
1.095
0.13093087
0
3
3852
101
162
952
57
115
0.2386
-2.067334052
0
4
537
87
152
459
60
115
0.8867
-0.173482019
0
5
1316
88
146
2700
57
112
2.152
1.105678078
0
6
3078
86
147
4525
63
125
1.491
0.576280258
0
7
12064
84
178
22286
60
153
1.855
0.891419187
0
8
4088
85
165
8892
59
128
2.207
1.14208663
0
9
1898
88
153
580
59
113
0.2878
-1.796861503
0
10
1085
93
151
1018
60
119
0.9657
-0.050353017
0
11
7225
101
165
5791
61
126
0.8043
-0.314194375
0
12
2028
86
153
2930
56
110
1.48
0.565597176
0
13
1526
108
170
916
58
114
0.6051
-0.72475451
0
14
3296
102
174
4146
63
127
1.278
0.353887836
0
15
6074
109
178
6094
62
126
1.011
0.015782997
0
16
1761
97
157
1846
60
117
1.073
0.101650076
0
17
1283
82
140
2290
57
116
1.859
0.89452677
0
18
3704
95
155
3368
60
117
0.9166
-0.125635809
0
19
1327
87
155
4182
58
111
3.326
1.733788169
0
20
4378
92
158
6114
64
123
1.412
0.497740089
0
4608 221 Kbytes .Supplementary data files not provided
