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Sample GSM2797193 Query DataSets for GSM2797193
Status Public on Aug 27, 2019
Title Ucp2-/- (KO) sample 6
Sample type RNA
 
Source name Ucp2-/- (KO) sample 6
Organism Mus musculus
Characteristics tissue: Colon tumors
strain: C57BL6/J
genotype: Ucp2-/- (KO)
Treatment protocol Mice were sacrificed at week 15 after starting AOM/DSS protocol and colon tumors were collected
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the miRNeasy Mini Kit according to the manufacturer's instructions.
Label biotin
Label protocol 240 ng of total RNA is reverse transcribed following the Gene chip® WT-Amp Plus kit (Affymetrix). Briefly, the resulting double stranded cDNA is used to produce single stranded RNA using T7 RNA pol. This cRNA is reverse transcribed in single stranded sens DNA which is fragmented and biotin labelled
 
Hybridization protocol biotynilated single stranded sense cDNA is then hybridized to GeneChip® Mouse Gene2.0 (Affymetrix) at 45°C for 17 hours
Scan protocol After overnight hybridization, chips are washed on the fluidic station FS450 following specific protocols (Affymetrix) and scanned using the GCS3000 7G
Data processing Data were normalized using RMA and statistical analysis was realized on Partek Genomics Suite. CDF file V20 was used for annotation.
 
Submission date Sep 28, 2017
Last update date Aug 27, 2019
Contact name Franck Letourneur
E-mail(s) franck.letourneur@inserm.fr
Organization name INSERM
Department U1016
Lab genomic
Street address 22 rue mechain
City Paris
ZIP/Postal code 75014
Country France
 
Platform ID GPL22598
Series (1)
GSE104403 Expression data in Ucp2+/+ (WT) and Ucp2-/- (KO) colon tumors from AOM/DSS-treated mice

Data table header descriptions
ID_REF
VALUE Log2-RMA signal

Data table
ID_REF VALUE
100009600_at 4.21472
100009609_at 2.9064
100009614_at 3.89513
100009664_at 3.63581
100012_at 3.07876
100017_at 7.75974
100019_at 7.67567
100033459_at 2.97242
100034251_at 7.43711
100034729_at 3.08799
100034739_at 3.99126
100036518_at 3.4423
100036520_at 5.56129
100036521_at 5.78384
100036523_at 6.53623
100036537_at 4.55425
100036768_at 5.06979
100037258_at 9.63368
100037260_at 4.37132
100037262_at 6.30762

Total number of rows: 23747

Table truncated, full table size 406 Kbytes.




Supplementary file Size Download File type/resource
GSM2797193_6-KO_262T_MoGene-2_0-st_.CEL.gz 9.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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