|
| Status |
Public on Aug 27, 2019 |
| Title |
Ucp2-/- (KO) sample 8 |
| Sample type |
RNA |
| |
|
| Source name |
Ucp2-/- (KO) sample 8
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Colon tumors
strain: C57BL6/J
genotype: Ucp2-/- (KO)
|
| Treatment protocol |
Mice were sacrificed at week 15 after starting AOM/DSS protocol and colon tumors were collected
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the miRNeasy Mini Kit according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
240 ng of total RNA is reverse transcribed following the Gene chip® WT-Amp Plus kit (Affymetrix). Briefly, the resulting double stranded cDNA is used to produce single stranded RNA using T7 RNA pol. This cRNA is reverse transcribed in single stranded sens DNA which is fragmented and biotin labelled
|
| |
|
| Hybridization protocol |
biotynilated single stranded sense cDNA is then hybridized to GeneChip® Mouse Gene2.0 (Affymetrix) at 45°C for 17 hours
|
| Scan protocol |
After overnight hybridization, chips are washed on the fluidic station FS450 following specific protocols (Affymetrix) and scanned using the GCS3000 7G
|
| Data processing |
Data were normalized using RMA and statistical analysis was realized on Partek Genomics Suite. CDF file V20 was used for annotation.
|
| |
|
| Submission date |
Sep 28, 2017 |
| Last update date |
Aug 27, 2019 |
| Contact name |
Franck Letourneur |
| E-mail(s) |
franck.letourneur@inserm.fr
|
| Organization name |
INSERM
|
| Department |
U1016
|
| Lab |
genomic
|
| Street address |
22 rue mechain
|
| City |
Paris |
| ZIP/Postal code |
75014 |
| Country |
France |
| |
|
| Platform ID |
GPL22598 |
| Series (1) |
| GSE104403 |
Expression data in Ucp2+/+ (WT) and Ucp2-/- (KO) colon tumors from AOM/DSS-treated mice |
|
