|
| Status |
Public on Dec 01, 2017 |
| Title |
G2-5 OBM |
| Sample type |
RNA |
| |
|
| Source name |
iPSc established by my lablatory
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: oral muccosal fibroblast
cell type: disease specific iPSc
treatment: After EB formation, sample was treated 10 day with OBM medium
|
| Treatment protocol |
Protocl was weritten in PloS one 2014 vol: 9 (6) pp: e99534
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with QIAZOL
|
| Label |
SYBR Green
|
| Label protocol |
PCR assays were performed using RT² Profiler™ PCR Array Human Hedgehog Signaling Pathway (PAHS-078Z, SA Biosciences, Frederick, MD) following the Manufacturer’s instructions. Reverse transcription was performed from 500 ng total RNA using the RT2 First Strand Kit (SA Biosciences). Quantitative real-time PCR were performed (ABI7500, ABI).
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
Autosomal dominant inheritance
|
| Data processing |
The web-based software package automatically performs all deltadeltaCt based fold-change calculations from the uploaded raw threshold cycle data.
Matrix normalized worksheet reports normalized signal (against housekeeping genes). Fold Change worksheet reports test/control ratios.
|
| |
|
| Submission date |
Oct 02, 2017 |
| Last update date |
Dec 01, 2017 |
| Contact name |
Shoko Onodera |
| Organization name |
Tokyo Dental collage
|
| Department |
biochemistry
|
| Street address |
2-9-18
|
| City |
chiyodaku misakichou |
| State/province |
Tokyo |
| ZIP/Postal code |
101-0061 |
| Country |
Japan |
| |
|
| Platform ID |
GPL24072 |
| Series (1) |
| GSE104499 |
Gorlin syndrome-derived induced pluripotent stem cells are hypersensitive to Hedgehog-mediated osteogenic induction |
|
