|
| Status |
Public on Jul 30, 2008 |
| Title |
BN21 4month high dose |
| Sample type |
RNA |
| |
|
| Source name |
rat liver from 4month Brown Norway exposed to toluene
|
| Organism |
Rattus norvegicus |
| Characteristics |
Male Brown Norway rats were obtained from Charles River through NIA.
no additional
|
| Treatment protocol |
1.0g/kg body weight toluene by gavage in corn oil
|
| Growth protocol |
Rats were housed individually. Animal facilities were controlled for temperature (20-24°C) and relative humidity (40-60%), and kept under a 12-h light-dark cycle.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the TriReagent procedure (Molecular Research Center, Cincinnati, OH) and the Qiagen Rneasy mini RNA cleanup protocol (Qiagen, Valencia, CA)
|
| Label |
biotin
|
| Label protocol |
Samples were labeled according to the Affymetrix protocol
|
| |
|
| Hybridization protocol |
Samples were hybridized according to the Affymetrix protocol
|
| Scan protocol |
Affymetrix protocol using a GeneChip scanner 3000
|
| Description |
gene expression data from rat liver samples- 4,12,24 months old
|
| Data processing |
Affymetrix GeneChip microarray suite software was used for washing and scanning. Expression summaries were calculated using an analysis algorithm adapted from Choe et al. (2005) [PMID: 15693945], in the sequence of "Background correction (MAS5), Quantile normalization (RMA), Perfect match adjustment (MAS5), Median polish (RMA), LOESS normalization.
|
| |
|
| Submission date |
Apr 08, 2008 |
| Last update date |
Jul 30, 2008 |
| Contact name |
Hongzu Ren |
| E-mail(s) |
ren.hongzu@epa.gov
|
| Phone |
919-541-4576
|
| Organization name |
US Environmental Protection Agency
|
| Lab |
NHEERL Toxicogenomics Core
|
| Street address |
109 TW Alexander Dr
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27711 |
| Country |
USA |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE11097 |
Coordinated Changes in Xenobiotic Metabolizing Enzyme Gene Expression in Aging Male Rats: Brown Norway and F344 |
|
