|
| Status |
Public on Mar 10, 2018 |
| Title |
8_denerv_2_mRNA |
| Sample type |
RNA |
| |
|
| Source name |
Denervated bladders from rats after bilat. pelvicus freezing
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague Dawley
gender: female
age: ~3 months
tissue: urinary bladder
in vivo treatments: surgery and bilateral ganglion freezing
tissue harvesting: euthanization
cell type: over 95percent smooth muscle
|
| Treatment protocol |
surgery (Sham samples) and surgery and bilateral ganglion freezing (for denerv samples)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
miRneasy plus RNeasy MinElute Cleanup
|
| Label |
Biotin Allonamide Triphosphate
|
| Label protocol |
Biotin Allonamide Triphosphate and label incorporation Labelling reaction at 37 degrees
|
| |
|
| Hybridization protocol |
Following fragmentation, 3.5ug of ssDNA were hybridized. See further information in chapter 3 (https://www.thermofisher.com/search/results?persona=DocSupport&refinementSearch=true&query=Manuals+%26+Protocols&navId=4294959596&refinementQuery=WT+PLUS+)
|
| Scan protocol |
GeneChips were scanned using the Genechip Scanner 3000. GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Data processing |
normalised using RMA method. CHP files extracted from Affymetrix Expression Console Software v1.4.1.46
|
| |
|
| Submission date |
Oct 03, 2017 |
| Last update date |
Mar 10, 2018 |
| Contact name |
Jessica Margareta Lindvall |
| E-mail(s) |
jessica.lindvall@nbis.se
|
| Organization name |
SciLifeLab. Stockholm University
|
| Department |
NBIS
|
| Street address |
Tomtebodavägen 23A
|
| City |
Solna |
| ZIP/Postal code |
11111 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL17117 |
| Series (2) |
| GSE104538 |
Gene activity induced by denervation in the rat urinary bladder [mRNA] |
| GSE104540 |
Gene activity induced by denervation in the rat urinary bladder |
|
