|
Status |
Public on Mar 10, 2018 |
Title |
10_denerv_4_miRNA |
Sample type |
RNA |
|
|
Source name |
Denervated bladders from rats after bilat. pelvicus freezing
|
Organism |
Rattus norvegicus |
Characteristics |
strain: Sprague Dawley gender: female age: ~3 months tissue: urinary bladder in vivo treatments: surgery and bilateral ganglion freezing tissue harvesting: euthanization cell type: over 95 percent smooth muscle
|
Treatment protocol |
miRneasy plus RNeasy MinElute Cleanup
|
Growth protocol |
surgery (Sham samples) and surgery and bilateral ganglion freezing (for denerv samples)
|
Extracted molecule |
total RNA |
Extraction protocol |
Biotin with Flash Tag Ligation
|
Label |
Biotin
|
Label protocol |
Protocol in chapter 3 was followed (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mirna_array_strip_flashtag_manual.pdf)
|
|
|
Hybridization protocol |
GeneChips were scanned using the Genechip Scanner 3000. GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
Scan protocol |
not provided
|
Description |
miRNA enriched
|
Data processing |
normalised using RMA method. CHP files extracted from Affymetrix Expression Console Software v1.4.1.46
|
|
|
Submission date |
Oct 03, 2017 |
Last update date |
Mar 10, 2018 |
Contact name |
Jessica Margareta Lindvall |
E-mail(s) |
jessica.lindvall@nbis.se
|
Organization name |
SciLifeLab. Stockholm University
|
Department |
NBIS
|
Street address |
Tomtebodavägen 23A
|
City |
Solna |
ZIP/Postal code |
11111 |
Country |
Sweden |
|
|
Platform ID |
GPL21572 |
Series (2) |
GSE104539 |
Gene activity induced by denervation in the rat urinary bladder [miRNA] |
GSE104540 |
Gene activity induced by denervation in the rat urinary bladder |
|