|
| Status |
Public on Sep 04, 2008 |
| Title |
Wild type mouse lungs treated with CCl4 |
| Sample type |
RNA |
| |
|
| Source name |
mouse lung
|
| Organism |
Mus musculus |
| Characteristics |
total lung RNA from mice treated with CCl4
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tissues were quickly removed and frozen in liquid nitrogen and stored at -80C for subsequent preparation of total RNA. Total RNA was isolated using TRI-Reagent (Molecular Research Center Inc. Cincinnati, Ohio) with modifications to remove DNA using the Qiagen RNAeasy columns and DNaseI Kit (Qiagen, Valencia, CA). RNA was stored at –70C in RNase-free H2O supplemented with the RNase inhibitor Superasin (Ambion, Austin, TX) as per manufacturers directions. Quality and quantity of RNA was determined using a Agilent Bioanalyzer as per manufacturers procedures (Agilent Technologies, Palo Alto, CA).
|
| Label |
DIG-UTP
|
| Label protocol |
1 ug of total RNA was used to transcribe DIG-labeled cRNA using Applied Biosystems Chemiluminescent RT-IVT Kit V2.0.
|
| |
|
| Hybridization protocol |
Microarray hybridization (using twenty micrograms of fragmented, DIG-labeled cRNA) and processing were performed according to Applied Biosystems protocols.
|
| Scan protocol |
Chemiluminescence detection, imaging, auto gridding, and image analysis was done according to Applied Biosystems protocols and the 1700 Chemiluminescent Microarray Analyzer Software v. 1.0.3.
|
| Description |
sample contained a mixture of equal amounts of RNA from three distinct WT mice, treated with CCl4 for 16 hours.
|
| Data processing |
Signal intensities across microarrays were normalized using the quantile-quantile method (www.bioconductor.org).
|
| |
|
| Submission date |
Apr 09, 2008 |
| Last update date |
Apr 11, 2008 |
| Contact name |
Tanya Kalin |
| Organization name |
University of Chicago
|
| Department |
Medicine
|
| Lab |
Pulmonary and Critical Care Medicine
|
| Street address |
5841 S. Maryland Ave.
|
| City |
Chicago |
| State/province |
IL |
| ZIP/Postal code |
60637 |
| Country |
USA |
| |
|
| Platform ID |
GPL2995 |
| Series (1) |
| GSE11112 |
Foxf1 enchances pulmonary inflammation and mastocytosis |
|
