|
| Status |
Public on Jan 31, 2019 |
| Title |
PB38_MSC_2_BRCA1+/- |
| Sample type |
RNA |
| |
|
| Source name |
mesenchymal derived from IPSCs BRCA1+/-
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: mesenchyme derived from peripheral blood IPSC
genotype: BRCA1+/-
|
| Growth protocol |
mesenchymal differentiation of human IPSCs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA with RNeasy Mini kit with DNase I digestion according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (Following the GeneChip whole transcript (WT) Sense Target labelling assay kit Affymetrix)
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cDNA were hybridized for 16 hr at 45C on GeneChip human Gene from Affymetrix GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GCS 3000 scanner (Affymetrix)
|
| Description |
BRCA1 haploinsufficiency
|
| Data processing |
the data were RMA normalized and analysed with Expression Console
|
| |
|
| Submission date |
Oct 06, 2017 |
| Last update date |
Jan 31, 2019 |
| Contact name |
christophe desterke |
| E-mail(s) |
christophe.desterke@inserm.fr
|
| Phone |
+33 (0)145595316
|
| Organization name |
university Paris Sud11
|
| Department |
UFR medecine institute Andre Lwoff
|
| Lab |
hopital Paul Brousse Batiment Lavoisier
|
| Street address |
14-16 avenue Paul Vaillant Couturier
|
| City |
VILLEJUIF |
| ZIP/Postal code |
94807 |
| Country |
France |
| |
|
| Platform ID |
GPL16686 |
| Series (1) |
| GSE104693 |
BRCA1 haploinsufficient Mesenchyme Derived from human peripheral blood Induced Pluripotent Stem Cell |
|
