|
| Status |
Public on Oct 01, 2019 |
| Title |
T,biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
bladder cancer T24 cells treated with piperlongumine
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: T24
cell type: bladder cancer cell line
treated with: Piperlongumine (PL)
|
| Treatment protocol |
T24 cells was subsequently treated with PL 20 μM or 0 μM (DMSO control)
|
| Growth protocol |
T24 cells in logarithmic growth phase were seeded in 60 mm dishes at a density of 4 × 104 cell/cm2 and incubated overnight.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
After drug treatment, total RNA was extracted from bladder cancer cell using Trizol reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
1000 ng of total RNA was labeled using Affymetrix Flashtag Biotin HSR RNA Labeling kit (P/N 901910, Affymetrix, Santa Clara, CA, USA) according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
900 ng of biotin labeled RNA was hybridized to Affymetrix GeneChip® miRNA 4.0 Arrays at 48 °C and 60 rpm for 16-18 h.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G.
|
| Description |
A1089
miRNA expression data from piperlongumine treated bladder cancer T24 cells
|
| Data processing |
Data were collected using Affymetrix Command Console Software.
|
| |
|
| Submission date |
Oct 08, 2017 |
| Last update date |
Oct 01, 2019 |
| Contact name |
Di Liu |
| E-mail(s) |
didiana@163.com
|
| Phone |
13507171375
|
| Organization name |
Union Hospital
|
| Department |
Department of Urology
|
| Street address |
1277 Jiefang Road
|
| City |
Wuhan |
| State/province |
Hubei |
| ZIP/Postal code |
430022 |
| Country |
China |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE104717 |
Expression data from piperlongumine treated bladder cancer T24 cells |
|
