|
| Status |
Public on Jan 15, 2018 |
| Title |
whole blood taken from sample 3162 |
| Sample type |
genomic |
| |
|
| Source name |
3162
|
| Organism |
Homo sapiens |
| Characteristics |
familyid: 316
twinid: 2
gender: F
zygosity: MZ
|
| Treatment protocol |
NA
|
| Growth protocol |
NA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from frozen whole blood samples using a standard cell lysis, proteinase K digestion, phenol/chloroform, ethanol precipitation method.
|
| Label |
Cy5 and Cy3
|
| Label protocol |
500 ng DNA from each sample was sodium bisulfite-treated using the EZ-96 DNA Methylation-Gol Kit (Zymo Research) according to the manufacturer’s standard protocol.
|
| |
|
| Hybridization protocol |
Samples were processed according to the Illumina Infinium HumanMethylation450K BeadChip (Illumina) protocol with the exception that 7ul of bisulfite treated material was input into the whole genome amplicifaction reaction instead of 4ul.
|
| Scan protocol |
Samples were assessed using the Illumina Infinium HumanMethylation450K BeadChip (Illumina) using a Illumina iScan System (Illumina).
|
| Description |
whole blood taken from sample 3162
|
| Data processing |
Data was imported using the R package methylumi methylumIDAT function. Data was then pre-processed in the R package wateRmelon using the pfilter and dasen functions.
Average Beta or normalized (provide the normalization method) Average Beta
|
| |
|
| Submission date |
Oct 16, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Jonathan Mill |
| Organization name |
University of Exeter
|
| Department |
Medical School
|
| Street address |
RILD Building, RD&E Hospital
|
| City |
Exeter |
| ZIP/Postal code |
EX2 5DW |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE105018 |
Whole blood DNA methylation profiles in participants of the Environmental Risk (E-Risk) Longitudinal Twin Study at age 18. |
|
