|
| Status |
Public on Jun 15, 2009 |
| Title |
tumor-reactive CD8+ T cell clone p26 |
| Sample type |
RNA |
| |
|
| Source name |
peripheral blood, tumor-reactive CD8+ T cell clone p26
|
| Organism |
Homo sapiens |
| Characteristics |
tumor-reactive CD8+ T cell clone
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated with Trizol(r) method according to the manufacturer's protocol. The RNA was purified with the RNeasy MiniElute Cleanup Kit (Qiagen, Hilden, Germany)
|
| Label |
biotin
|
| Label protocol |
50 to 100 nanograms of total RNA was used to generate double-stranded cDNA with a T7(dT)24-oligonucleotide primer performed with the One Cycle cDNA Synthesis Kit (Affymetrix, Santa Clara, CA, USA) followed by a second IVT reaction to amplify the cDNA. After purification with the Sample Cleanup Module (Affymetrix), cDNA served as a template to prepare biotinylated cRNA via IVT, using the IVT Labeling Kit (Affymetrix). The labeled cRNA transcripts were purified using the Sample Cleanup Module.
|
| |
|
| Hybridization protocol |
Fragmentation of cRNA transcripts and hybridization of the high-density oligonucleotide microarrays (HG-U133A arrays, Affymetrix) were performed according to the manufacturer's GeneChips Expression Analysis Technical Manual (Affymetrix).
|
| Scan protocol |
Scanning of the high-density oligonucleotide microarrays (HG-U133A arrays, Affymetrix) were performed according to the manufacturer's GeneChips Expression Analysis Technical Manual (Affymetrix).
|
| Description |
tumor-reactive CD8+ T cell clone p26
|
| Data processing |
Data has been normalized using dChip software
|
| |
|
| Submission date |
Apr 16, 2008 |
| Last update date |
Sep 01, 2016 |
| Contact name |
Joachim Schultze |
| E-mail(s) |
j.schultze@uni-bonn.de
|
| Organization name |
LIMES (Life and Medical Sciences Center Genomics and Immunoregulation)
|
| Department |
Genomics and Immunoregulation
|
| Street address |
Carl-Troll-Strasse 31
|
| City |
Bonn |
| State/province |
NRW |
| ZIP/Postal code |
53115 |
| Country |
Germany |
| |
|
| Platform ID |
GPL96 |
| Series (1) |
| GSE11188 |
Gene-expression profiles of non-tumor-reactive CD8+ T cells |
|
| Relations |
| Reanalyzed by |
GSE86363 |
