|
| Status |
Public on Oct 20, 2017 |
| Title |
WT_Kb-OVA+_CD8+_T_CELLS_REP2 |
| Sample type |
RNA |
| |
|
| Source name |
wild-type
|
| Organism |
Mus musculus |
| Characteristics |
genotype: wild-type
cell type: activated CD8 T cells
|
| Growth protocol |
Naïve littermate and Suv39h1-KO CD8+ T cells, and day 7 post-infection Kb-OVA+ CD8+ T cells (from Listeria .m-OVA infected mice), were isolated by flowcytometry
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Rneasy Micro Kit (QUIGEN) according to the manufacturer protocol. A column DNAse treatment was included (QUIGEN).
|
| Label |
biotin
|
| Label protocol |
For each condition, RNA was employed to synthetize cDNA according to the standard Affymetrix protocol.Affymetrix GeneTitan device.
|
| |
|
| Hybridization protocol |
Labelled DNA was hybrized on the Affymetrix Mouse Gene 2.1 ST processed on an Affymetrix GeneTitan device.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
Wild-type, activated CD8 T cells, biological rep2
|
| Data processing |
Microarray data were carried out with R (version 3.1.0) using packages from the Bioconductor. Raw data CEL files were used and the quality control analysis was performed using ArrayQualityMetrics package. The raw data were preprocessed using the RMA (Robust Multi-array Average) method available in oligo package. Gene expression levels were analyzed on a base-2 logarithmic scale.
|
| |
|
| Submission date |
Oct 19, 2017 |
| Last update date |
Jan 23, 2018 |
| Contact name |
Luigia Pace |
| E-mail(s) |
Luigia.Pace@curie.fr
|
| Organization name |
Institut Curie
|
| Street address |
26 rue d'Ulm
|
| City |
Paris |
| ZIP/Postal code |
75005 |
| Country |
France |
| |
|
| Platform ID |
GPL17400 |
| Series (1) |
| GSE105163 |
Transcriptome analysis of naive and Listeria monocytogenes-specific CD8+ T cells |
|
