|
| Status |
Public on Jun 20, 2008 |
| Title |
Col_BRZ_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
Col on BRZ
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
4-day-old, etiolated Col seedlings grown on 0.5 µM BRZ
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with Spectrum™ Plant Total RNA Kit from Sigma-Aldrich according to manufacturer’s protocols.
|
| Label |
biotin
|
| Label protocol |
Total RNA from the treated seedlings was used for preparing probes for the microarray experiments, which were carried out according to the protocols provided by the gene chip manufacturer Affymetrix (Santa Clara, California, United States).
|
| |
|
| Hybridization protocol |
Hybridization and washing were were carried out according to the protocols provided by the gene chip manufacturer Affymetrix (Santa Clara, California, United States).
|
| Scan protocol |
Scan protocols were were carried out according to the protocols provided by the gene chip manufacturer Affymetrix (Santa Clara, California, United States).
|
| Description |
Total RNA was extracted from 4-day-old, etiolated arf2 and Col Arabidopsis seedlings grown on 0.5 µM BRZ or mock treatments.
|
| Data processing |
Expression was normalized and estimated using the gcRMA package of BioConductor.
|
| |
|
| Submission date |
Apr 20, 2008 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Jennifer Nemhauser |
| E-mail(s) |
jn7@u.washington.edu
|
| Organization name |
Univ. of Washington
|
| Street address |
box 351800
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98195-1800 |
| Country |
USA |
| |
|
| Platform ID |
GPL198 |
| Series (1) |
| GSE11216 |
Brassinazole treatment of arf2 and wild-type dark-grown seedlings |
|
| Relations |
| Reanalyzed by |
GSE119083 |
