|
| Status |
Public on Nov 10, 2017 |
| Title |
LS174T_K1_2 |
| Sample type |
RNA |
| |
|
| Source name |
LS174T cell clone (2F8),CRISPR/Cas9 treated, FOXA1 Knock-out
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LS174T
replicate: 2
genotype: FOXA1 Knockout
treatment: transfected
|
| Treatment protocol |
LS174T cells were subjected to CRISPR/Cas9 genome editing using a guide RNA targeting exon 2 of the FOXA1 locus, followed by single cell cloning.
|
| Growth protocol |
LS174T cells were purchased from the Cell Line Service culture collection (CLS #300392, DKFZ, Heidelberg). Cell lines were maintained in growth medium consisting of DMEM supplemented with 10% FCS, 10 mM Hepes, 1/100 MEM non-essential amino acid solution and 1/100 penicillin/streptomycin
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from LS174T cells using the PeqGOLD total RNA Kit (#732-2871, Peqlab/VWR Life Science)
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
K1_2
Array ID: 200739030022
|
| Data processing |
All arrays were normalized together using the quantile normalization algorithm without background subtraction using the R Bioconductor beadarray package
|
| |
|
| Submission date |
Oct 24, 2017 |
| Last update date |
Nov 10, 2017 |
| Contact name |
Hauke Busch |
| E-mail(s) |
hauke.busch@uni-luebeck.de
|
| Phone |
+49-451-3101-8470
|
| Organization name |
University of Lübeck
|
| Department |
Lübeck Institute of Experimental Dermatology
|
| Street address |
Ratzeburger Allee 160
|
| City |
Lübeck |
| State/province |
Schleswig-Holstein |
| ZIP/Postal code |
23538 |
| Country |
Germany |
| |
|
| Platform ID |
GPL10558 |
| Series (1) |
| GSE106073 |
SNAIL1-mediated Downregulation of FOXA Proteins Facilitates the Inactivation of Transcriptional Enhancer Elements at Key Epithelial Genes in Colorectal Cancer Cells |
|
