|
| Status |
Public on Jul 10, 2018 |
| Title |
NCI-H209 siTTF-1 |
| Sample type |
RNA |
| |
|
| Source name |
human small cell lung cancer cell line NCI-H209 electroporated with siRNAs for TTF-1 (siTTF-1)
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: NCI-H209
transfection: siTTF1
|
| Treatment protocol |
NCI-H209 cells (1 × 107) were mixed with 1 μM siRNAs in an electroporation cuvette and electroporated using Gene Pulser II Electroporation Systems (Bio-Rad, Hercules, CA, USA) with voltage 200 V and capacitance 950 μF.
|
| Growth protocol |
NCI-H209 cells were cultured in RPMI1640 + 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted 48 h after siNC or siTTF-1 transfection, using RNeasy Mini Kit (Qiagen, Hilden, Germany). Total RNA QC was performed by Agilent 2100 Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
Biotin-labeled samples were prepared using FlashTagTM Biotin HSR RNA Labeling Kit for Affymetrix GeneChip miRNA Arrays (Affymetrix, Inc., cat.# 901911) .
|
| |
|
| Hybridization protocol |
Hybridization was performed with Hybridization Control Kit (Affymetrix, Inc., cat.# 900454) and Hybridization, Wash, and Stain Kit (Affymetrix, Inc., cat.# 900720) in GeneChip Hybridization Oven 645 (Affymetrix, Inc.) for 18 hr (60 rpm) at 48 degree celsius. Washing was performed using GeneChip Fluidics Station 450 (Affymetrix, Inc.).
|
| Scan protocol |
Slides were scanned using GeneChip® Scanner 3000 7G
|
| Data processing |
Images were digitized with Affymetrix® Expression Console™ Software.
|
| |
|
| Submission date |
Oct 25, 2017 |
| Last update date |
Jul 10, 2018 |
| Contact name |
Masafumi Horie |
| E-mail(s) |
mhorie-tky@umin.ac.jp
|
| Organization name |
University of Southern California
|
| Street address |
2011 Zonal Ave
|
| City |
Los Angeles |
| ZIP/Postal code |
90033 |
| Country |
USA |
| |
|
| Platform ID |
GPL21572 |
| Series (1) |
| GSE106164 |
miRNAs regulated by TTF-1 in small cell lung cancer cell lines |
|
