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Sample GSM283120

Query DataSets for GSM283120

Status

Public on Nov 17, 2008

Title

zld-GCL 1-2 hr 2

Sample type

RNA

Source name

zld germ-line colne, 1 to 2 hr embryo

Organism

Drosophila melanogaster

Characteristics

zld germ-line clone, 1 to 2 hr embryo

Extracted molecule

total RNA

Extraction protocol

1-2 hr embryonic total RNA samples were extracted form duplicate collections of yw and zld-glc embryos by TRIzol (invitrogen).

Label

Biotin

Label protocol

cDNA was prepared by GeneChip® HT One-Cycle cDNA Synthesis Kit (Manufactured by Invitrogen for Affymetrix) and labeled by BioArray™ HighYield™ RNA Transcript Labeling Kit (Enzo)

Hybridization protocol

standard Affymetrix procedures

Scan protocol

standard Affymetrix procedures

Description

zld-glc 1-2 hr embryo

Data processing

standard Affymetrix procedures

Submission date

Apr 21, 2008

Last update date

Aug 28, 2018

Contact name

Christine Rushlow

Organization name

NYU

Department

Biology

Street address

100 Washington Sq.E.

City

New York

State/province

ZIP/Postal code

10003

Country

USA

Platform ID

GPL1322

Series (1)

GSE11231

The zinc finger protein Zelda plays a key role in the maternal to zygotic transition in Drosophila

Relations

Reanalyzed by

GSE119084

Data table header descriptions
ID_REF
VALUE	GCOS-calculated Signal intensity
ABS_CALL	an absolute analysis that indicates if the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE	detection p-value indicates the significance level of the detection call

Data table
ID_REF	VALUE	ABS_CALL	DETECTION P-VALUE
AFFX-BioB-5_at	78	P	0.015183
AFFX-BioB-M_at	51.9	P	0.000754
AFFX-BioB-3_at	24.9	A	0.185131
AFFX-BioC-5_at	122.1	P	0.000662
AFFX-BioC-3_at	89.4	P	0.000754
AFFX-BioDn-5_at	244.6	P	0.000044
AFFX-BioDn-3_at	629.4	P	0.000662
AFFX-CreX-5_at	1548.9	P	0.00007
AFFX-CreX-3_at	1506.8	P	0.000044
AFFX-DapX-5_at	1.5	A	0.559354
AFFX-DapX-M_at	7.6	A	0.470241
AFFX-DapX-3_at	1.8	A	0.794268
AFFX-LysX-5_at	3.7	A	0.147939
AFFX-LysX-M_at	5	A	0.712257
AFFX-LysX-3_at	7.6	M	0.062929
AFFX-PheX-5_at	2	A	0.724854
AFFX-PheX-M_at	1.4	A	0.843268
AFFX-PheX-3_at	8.2	A	0.58862
AFFX-ThrX-5_at	13.7	A	0.262827
AFFX-ThrX-M_at	1.4	A	0.659339

Total number of rows: 18952

Table truncated, full table size 503 Kbytes.

Supplementary file	Size	Download	File type/resource
GSM283120.CEL.gz	2.4 Mb	(ftp)(http)	CEL
Processed data included within Sample table