|
Status |
Public on Sep 05, 2018 |
Title |
09027: Saline-Control 2 |
Sample type |
SRA |
|
|
Source name |
Isolated fetal pancreatic islets
|
Organism |
Ovis aries |
Characteristics |
tissue: Isolated fetal pancreatic islets gestational age (days): 139 fetal sex: female fetal weight (kg): 3.6
|
Treatment protocol |
Control fetuses received an intravenous infusion of diluent (0.3% ascorbic acid in 0.9% saline). NE fetuses received an intravenous infusion of NE (Norepinephrine Bitartrate, Bedford Labs) for 7 days at preset rates for each day of treatment (day 1 at 1 µg/min, days 2-4 at 2 µg/min, and days 5-7 at 4µg/min).
|
Growth protocol |
Columbia-Rambouillet crossbred ewes carrying singleton pregnancies.
|
Extracted molecule |
total RNA |
Extraction protocol |
Islets were isolated and handpicked then frozen at -80C for RNA extraction with Qiagen Microprep RNAeasy mRNA was selected and double-stranded cDNA libraries with ligated sequencing adapters was constructed with the Illumina TruSeq RNA Sample Prep Kit. Cluster generation was conducted with Illumina TruSeq 100bp PE (paired end) cluster kit
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|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Description |
09-027
|
Data processing |
Tophat (v2.0.1) -a 15 -m 1 -I 50000 -g 9 -N 3 -rgl 4 --red 7 -rred 0 cufflinks (v2.1.1) -G -b oar3174.fa -u -N -F 0.05 Annotation- Ensembl Ovis_aries.Oar_v3.1.74.gtf cuffmerge -G cuffdiff -N -u -c 24 --library-type fr-unstranded -b oar3174.fa --max-bundle-frags 25000000 Genome_build: Ensemble release 74 Ovis_aries.Oar_v3.1.dna_sm.toplevel.fa.gz Supplementary_files_format_and_content: gene_exp.diff - cuffdiff gene level statistics Supplementary_files_format_and_content: gene_id.repFPKM.txt - table of gene ID, locus and FPKM values from cuffdiff genes.FPKM_tracking
|
|
|
Submission date |
Nov 02, 2017 |
Last update date |
May 15, 2019 |
Contact name |
Sean W Limesand |
E-mail(s) |
limesand@ag.arizona.edu
|
Phone |
520-626-8903
|
Organization name |
University of Ariziona
|
Department |
Animal & Comparative Biomedical Sciences
|
Lab |
Perinatal Biology Laboratory
|
Street address |
4101 N Campbell Ave
|
City |
Tucson |
State/province |
AZ |
ZIP/Postal code |
85719 |
Country |
USA |
|
|
Platform ID |
GPL19778 |
Series (1) |
GSE106440 |
RNAseq of Islets Isolated from Near Term Fetal Sheep after 7-day Infusion of Norepinephrine or Vehicle Control |
|
Relations |
BioSample |
SAMN07967135 |
SRA |
SRX3353293 |