|
| Status |
Public on Nov 20, 2011 |
| Title |
M833 / Reference_13425475 |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
M833
|
| Organism |
Mus musculus |
| Characteristics |
M833
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy3
|
| Label protocol |
Direct incorporation of CyDye labeled dCTP by reverse transcription.
|
| |
|
| Channel 2 |
| Source name |
ref
|
| Organism |
Mus musculus |
| Characteristics |
ref
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol
|
| Label |
Cy5
|
| Label protocol |
Direct incorporation of CyDye labeled dCTP by reverse transcription.
|
| |
|
| |
| Hybridization protocol |
Hybridization was performed at 50C and 35% formamide for 16 hours.
|
| Scan protocol |
Microarrays were scanned using the Agilent G2505B scanner.
|
| Description |
Gene expression data from cell type: M833
|
| Data processing |
Image files were analyzed by use of GenePix Pro 4 image analysis software (Axon Instruments). The raw data was preprocessed using a pin-tip dependent normalization method as implemented in the limma package within Bioconductor. Calculated log2 ratio of Cy5/Cy3. VALUE column represents log2(test/ref) ratios.
|
| |
|
| Submission date |
May 01, 2008 |
| Last update date |
Nov 20, 2011 |
| Contact name |
Karl Dykema |
| Organization name |
Van Andel Institute
|
| Lab |
Lab of Computational Biology
|
| Street address |
333 Bostwick Ave. NE
|
| City |
Grand Rapids |
| State/province |
MI |
| ZIP/Postal code |
49503 |
| Country |
USA |
| |
|
| Platform ID |
GPL4487 |
| Series (1) |
|
