|
| Status |
Public on May 13, 2008 |
| Title |
Slide 15 P stage (fully penetrated) 4th nymphs by the parasitoid |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
4th nymphs fully penetrated by the parasitoid
|
| Organism |
Bemisia tabaci |
| Characteristics |
B. tabaci 4th nymphs fully penetrated by the parasitoid
|
| Treatment protocol |
Full penetration by the parasitoid E. mundus
|
| Growth protocol |
N/A
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
TRIZOL
|
| Label |
Cy5
|
| Label protocol |
standard Aminoallyl
|
| |
|
| Channel 2 |
| Source name |
4th nymphs control non parasitized
|
| Organism |
Bemisia tabaci |
| Characteristics |
4th B. tabaci nymphs non parasitized as a control
|
| Treatment protocol |
4th nymphs non parasitized
|
| Growth protocol |
N/A
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
TRIZOL
|
| Label |
Cy3
|
| Label protocol |
Aminoallyl
|
| |
|
| |
| Hybridization protocol |
standard Aminoallyl
|
| Scan protocol |
Agilent automatic scanner
|
| Description |
N/A
|
| Data processing |
LOWESS normalization method was used for each channel separately.
genepix was used for data acquisition
numerator is a sample from fully penetrated nymphs as the treatment labeled with Cy5.
denominator is a sample from non parasitized nymphs as a control labeled with Cy3.
numbers in the VALUE column are therefore normalized data and the outcome is the Cy5/Cy3 ratio after log2 transformation.
|
| |
|
| Submission date |
May 10, 2008 |
| Last update date |
May 12, 2008 |
| Contact name |
Murad Ghanim |
| E-mail(s) |
ghanim@agri.gov.il
|
| Phone |
+ 972 3 9683911
|
| Fax |
+ 972 3 9683445
|
| URL |
http://www.agri.gov.il/en/people/759.aspx
|
| Organization name |
Volcani Center
|
| Department |
Entomology
|
| Lab |
Ghanim
|
| Street address |
Hamacabim
|
| City |
Bet Dagan |
| ZIP/Postal code |
50250 |
| Country |
Israel |
| |
|
| Platform ID |
GPL6850 |
| Series (1) |
| GSE11410 |
Parasitization of Bemisia tabaci by Eretmocerus mundus |
|
