|
| Status |
Public on Mar 31, 2009 |
| Title |
NPC_corticosterone_2 |
| Sample type |
RNA |
| |
|
| Source name |
NPC treated with 1 uM corticosterone for 48 hr
|
| Organism |
Rattus norvegicus |
| Characteristics |
Wister
NPC from cerebral cortices of embryo at E15.5
|
| Treatment protocol |
Cells were treated with 1 uM corticosterone or vehicle (DMSO) for 48 hr.
|
| Growth protocol |
Neuronal progenitor cells were prepared from cerebral cortices of rat embryo at E15.5. Cells were cultured with 20 ng/ml bFGF on fibronectin coated dish.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using TRIzol reagent (Invitrogen). Quality of the RNA was certificated using Agilent 2100 Bioanalyzer (Agilent Technlogies).
|
| Label |
biotin
|
| Label protocol |
cDNA were synthesized by GeneChip T7-Oligo(dT) Promoter Primer Kit（Affymetrix) and TaKaRa cDNA Synthesis Kit (TaKaRa Bio) from 3ug total RNA. Biotinylated cRNA were synthesized by IVT Labeling Kit (Affymetrix) .
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Rat Genome 230 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000 7G.
|
| Description |
gene expression data from rat NPC treated with corticosterone
|
| Data processing |
Single Array Analysis were calculated by Microarray Suite version 5.0 (MAS5.0) with Affymetrix default setting and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
May 16, 2008 |
| Last update date |
Dec 24, 2008 |
| Contact name |
Taira Mayanagi |
| E-mail(s) |
taira@nbiochem.med.osaka-u.ac.jp
|
| Organization name |
Osaka University Graduate School of Medicine
|
| Street address |
Yamadaoka 2-2
|
| City |
Suita |
| State/province |
Osaka |
| ZIP/Postal code |
565-0871 |
| Country |
Japan |
| |
|
| Platform ID |
GPL1355 |
| Series (1) |
| GSE11478 |
Effect of corticosterone treatment on rat neuronal progenitor cells |
|
