|
| Status |
Public on Oct 05, 2018 |
| Title |
LNCap shTRIM28-1 |
| Sample type |
RNA |
| |
|
| Source name |
Prostate cancer cell line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: LNCaP
genotype: WT
|
| Treatment protocol |
LNCaP infected with pLKO, shTRIM28 or shTRIM24 were hormone starved for 3 days before treatment with ethanol or 1nM R1881 for 48h. LNCaP cells were infected with lentivirus harboring pLKO, shTRIM24, shTRIM28, or shTRIM28 and TRIM24
|
| Growth protocol |
Cells were maintained in RPMI supplemented with 10% FBS and 1% PS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from cells using PureLinkTM RNA Mini Kit (Life Tech). The quantity and quality of the isolated RNAs was determined using ND-1000 Spectrophotometer.
|
| Label |
biotin
|
| Label protocol |
The arrays were processed at University of Chicago Genomics Core (Illinois) according to manufacturer’s protocol.
|
| |
|
| Hybridization protocol |
The arrays were processed at University of Chicago Genomics Core (Illinois) according to manufacturer’s protocol.
|
| Scan protocol |
The arrays were processed at University of Chicago Genomics Core (Illinois) according to manufacturer’s protocol.
|
| Description |
SAMPLE 15
|
| Data processing |
The data were processed and normalised using Illumina GenomeStudio
|
| |
|
| Submission date |
Dec 15, 2017 |
| Last update date |
Oct 05, 2018 |
| Contact name |
Jindan Yu |
| E-mail(s) |
jindan.yu@emory.edu
|
| Organization name |
Emory University
|
| Department |
Urology
|
| Lab |
Jindan Yu's lab
|
| Street address |
E330, 1760 Haygood Dr NE
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30322 |
| Country |
USA |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE108145 |
TRIM28 protects TRIM24 from SPOP-mediated degradation and promotes prostate cancer progression [microarray] |
| GSE108146 |
TRIM28 protects TRIM24 from SPOP-mediated degradation and promotes prostate cancer progression |
|
