|
| Status |
Public on Feb 12, 2018 |
| Title |
L140-d726 |
| Sample type |
SRA |
| |
|
| Source name |
Seminal exosomes
|
| Organism |
Equus caballus |
| Characteristics |
infection: Equiena arteritis virus (long-term, persistent infection)
time: 726 days post-infection
|
| Treatment protocol |
Seminal exosomes were isolated using the ExoQuick Exosome Precipitation Solution (System Biosciences, Palo Alto, CA) from seminal plasma samples derived from the stallions at the specific time-points post-infection as described
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Exosomal RNA was extracted using the SeraMir Exosome RNA Isolation kit (System Biosciences, Palo Alto, CA)
cDNA libraries were prepared using TailorMix Micro RNA Sample Preparation version 2 (SeqMatic LLC, Fremont, CA) and perfomed by a commercial company (System Biosciences, Palo Alto, CA). cDNA libraries were size-selected on an 8% Tris-borate-EDTA (TBE) polyacrylamide gel
cDNA libraries were sequenced (1x75bp) in a Illumina NextSeq 500 platform.
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
bcl2Fastq
TrimGalore
mirDeep2
Genome_build: GCA_000002305.1
Supplementary_files_format_and_content: miRDeep2 csv result output
|
| |
|
| Submission date |
Dec 18, 2017 |
| Last update date |
Feb 12, 2018 |
| Contact name |
Theodore Samuel Kalbfleisch |
| E-mail(s) |
ted.kalbfleisch@louisville.edu
|
| Phone |
502-852-7495
|
| Organization name |
University of Louisville
|
| Department |
Biochemistry and Molecular Genetics
|
| Lab |
Kalbfleisch
|
| Street address |
221F Baxter II, 580 S Preston St
|
| City |
Louisville |
| State/province |
KY |
| ZIP/Postal code |
40202 |
| Country |
USA |
| |
|
| Platform ID |
GPL21401 |
| Series (1) |
| GSE108180 |
Downregulation of eca-mir-128 in seminal exosomes and enhanced expression of CXCL16 in the stallion reproductive tract are associated with equine arteritis virus long-term persistent infection |
|
| Relations |
| BioSample |
SAMN08196560 |
| SRA |
SRX3478268 |
