|
| Status |
Public on Dec 31, 2019 |
| Title |
ercDC_RCC_66 |
| Sample type |
RNA |
| |
|
| Source name |
ercDCs FACS-sorted from ccRCC tissue suspension
|
| Organism |
Homo sapiens |
| Characteristics |
donor/patient: 66
disease status: clear cell renal cell carcinoma
cell type: ercDCs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was prepared using RNeasy micro kit (Qiagen). RNA quantity and quality were assessed using a Nanodrop 1000 Spectrometer (Peqlab) and Agilent 2100 Bioanalyzer. RNA (0.5–15 ng) was amplified and converted into cDNA by a linear amplification method using WT-Ovation PicoSL System in combination with the Encore® Biotin Module (both from NuGen). cDNA was further purified using MinElute Reaction Cleanup kit (Qiagen) before hybridization to Affymetrix GeneChip® Human Gene ST 1.0 Arrays.
|
| Label |
biotin
|
| Label protocol |
Standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol.
|
| Scan protocol |
Standard Affymetrix protocol.
|
| Description |
ercDCs FACS-sorted from ccRCC tissue suspension of patient 66; gated as CD209+CD14+ cells among CD45+, live, single, CD11c+ CD3- cells.
|
| Data processing |
R/Bioconductor, rma normalization, default parameters.
|
| |
|
| Submission date |
Dec 19, 2017 |
| Last update date |
Dec 31, 2019 |
| Contact name |
Tobias Straub |
| E-mail(s) |
tstraub@med.uni-muenchen.de
|
| Organization name |
LMU Munich
|
| Department |
Biomedical Center, Bioinformatics
|
| Street address |
Großhadener Str. 9
|
| City |
Martinsried |
| ZIP/Postal code |
82152 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6244 |
| Series (2) |
| GSE108310 |
A mosaic renal myeloid subtype linked to immune escape correlates with poor patient survival in clear cell renal cell carcinoma [ex vivo] |
| GSE108312 |
A mosaic renal myeloid subtype linked to immune escape correlates with poor patient survival in clear cell renal cell carcinoma |
|
