|
| Status |
Public on Jun 19, 2018 |
| Title |
blood-HD-NS00025626-batch2-rep1 |
| Sample type |
RNA |
| |
|
| Source name |
HD_blood
|
| Organism |
Homo sapiens |
| Characteristics |
diagnosis: HD
gender: famale
age: 52.62
cap score: 386.2
cap_d_group: high
tissue: blood
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Using PAXgene Blood RNA Kit(PreAnalytiX Cat#:762164) for RNA extraction following the manufaturer's protocol.
|
| Label |
miRNA-specific probes
|
| Label protocol |
Not applicable
|
| |
|
| Hybridization protocol |
250 ng of total RNA was obtained from 233 samples, and hybridized to the nCounter Human miRNA sample probes (Seattle, WA).
|
| Scan protocol |
Each sample was scanned for 600 FOV on the nCounter Digital Analyzer
|
| Description |
Nanostring miRNA measurement for blood sample
NB00000097
|
| Data processing |
We first loaded raw RCC files for 233 samples into nSolver (v2.5) and extracted the raw counts, then substracted background noise based on mean of negative controls, normalized to positive controls, finally performed global normalization using all miRNAs.
|
| |
|
| Submission date |
Dec 21, 2017 |
| Last update date |
Jun 19, 2018 |
| Contact name |
Xianjun Dong |
| E-mail(s) |
xdong1@bwh.harvard.edu
|
| Organization name |
Brigham and Women's Hospital
|
| Department |
Neurology
|
| Lab |
Dong Lab
|
| Street address |
60 Fenwood Rd
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL17904 |
| Series (2) |
| GSE108395 |
Differential expression analysis of miRNAs expressed in blood and CSF of Huntington's disease [miRNA array] |
| GSE108398 |
Differential expression analysis of miRNAs expressed in blood and CSF of Huntington's disease |
|
